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Immunocytochemistry Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 10 min at 20 C, washed three times with PBS, and then incubated with blocking buffer (1% FBS and 1% BSA in PBS) for 1 h at 20 C

10.1016/j.neuropharm.2011.07.008 Neuropharmacology 208 WernekeU.HornO.TaylorD