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For cell cycle analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h

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When their seminal research was published in *Cell* in March 2017, it introduced the first precision senolytic peptide capable of selectively eliminating senescent cells while sparing healthy tissue, opening unprecedented opportunities for studying aging mechanisms and developing research tools for age-related cellular dysfunction

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