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To maximize the effectiveness of pre-workout supplements containing compounds like creatine, it is advisable to begin taking them well ahead of your workout

These include increased expression of glutamate cysteine ligase (also referred to as -glutamylcysteine synthetase) to promote GSH synthesis (272), upregulation of glutaredoxins that catalyze the reduction of S-glutathionylated proteins in the mitochondrial membrane (22), and increased expression and activation of the transcription factor, nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which regulates the expression of several genes essential for GSH synthesis and homeostasis (188)

XYC, QZ, JG participated in the study design and manuscript revising

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