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In 2013, in vivo -like pathophysiology studies were carried out cultivating primary commercial human proximal tubular epithelial cells on an OoC exploiting it for a toxicity study (165)

A major cause of CVD is atherosclerosis, a chronic inflammatory disease characterized by the pathogenic accumulation of vascular smooth muscle cells (VSMC), oxidized lipids and immune cells in the artery wall 2,3,4

Blots were then probed with the relevant primary antibodies in 5% BSA, 0.1% Tween-20 and 1 PBS or in 5% non-fat skimmed milk powder in 0.1% Tween-20 and 1 PBS at 4 C overnight with gentle motion in a hand-sealed transparent plastic bag

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