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Ozempic (semaglutide) is a popular GLP-1 drug used for diabetes and weight loss, and its use has grown rapidly in recent years

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Baseline Biomarker Assessment: The Foundation for Safe Dual Therapy Before starting testosterone with a GLP-1 medication, comprehensive baseline bloodwork should include: testosterone, estradiol, FSH/LH, fasting glucose, HbA1c, lipid panel, liver and kidney function, hematocrit, and thyroid markers

The cloning reaction was subsequently transformed into E.coli TOP10 cells (Life Technologies) for selection