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Cleavage Under Targets and Release Using Nuclease (CUT&RUN) The CUT&RUN protocol was adopted from the Nature Protocols manuscript by Skene, Henikoff & Henikoff 130,131

Using the HEK293-GCaMP6S cell line, we found that treatment with 5 M Hld Se induced a strong increase in cytoplasmic calcium, as shown by the levels of green fluorescence signal following exposure to the peptide and the Hld Se -induced increase in calcium levels appeared to be greater and more stable than the calcium response to Hld Sa (Fig

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