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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

GHK-Cu are widely used in anti-aging and repair products in the field of skincare and have the following effects: Promote collagen production: GHK-Cu can stimulate the formation of collagen and elastin, reduce the depth of wrinkles and spots, increase skin thickness, and tighten the skin

(Again this is not medical advice!) In addition to the vanity side of things, I want to say that there are also some exciting studies around this peptide as it pertains to brain health and to its anti-cancer properties