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Although not a direct fat loss peptide, it influences appetite and central nervous system pathways that intersect with metabolic signaling, which has led to some off-label discussion in weight management circles, along with further research

Growth media was replaced with stimulation buffer [phenol-free DMEM containing 0.1% (w/v) bovine serum albumin (BSA) and 1 mM 3-isobutyl-1-methylxanthine] and incubated for 1 h at 37 C in 5% CO 2

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