Packaged in a hygienic airless syringe pump, each ampoule ensures freshness, precision, and waste-free application
Subsequently, images were collected with CLSM (LSM-800, ZEISS, Germany) (DAPI, ex = 405 nm, em = 460 nm, Cy5.5, ex = 673 nm, em = 692 nm)
To explore this idea, we bred the MMTV-PyMT transgenic mouse strain (which spontaneously develops breast tumours) 26 with an uninduced Gclc f/f Rosa26 creERT2 mouse 27 in which the rate-limiting step in GSH synthesis, the glutamatecysteine ligase catalytic subunit (GCLC), can be knocked out throughout the body after induction with tamoxifen
Once synthesized, GSH serves as a substrate for GSH peroxidases (GPx), which reduce hydrogen peroxide and lipid peroxides into water and lipid alcohols, respectively (Vairetti et al., 2021)