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The cortical glutamate connections can be divided broadly into five major arcs that include PFC to the brainstem, PFC to the striatum and NAc, cerebral cortex to the thalamus, intracortical glutamate projections, and from the thalamus to the cerebral cortex (Figure 1) ( Neurometabolites Homeostasis in Healthy Brain Neurometabolites homeostasis plays a very important role in brain function, and has been shown to be affected in animal models and human subjects of various neuropsychiatric disorders including MDD ( In addition to precursors for different metabolites, these molecules play various critical functions that include signal transduction, osmoregulation, cell growth and protein synthesis ( in vivo by different MR spectroscopic approaches in human (Figure 2)

Total protein concentration in the supernatant was measured using BCA protein assay kit (Pierce Biotechnology)

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