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As such, the interfacial residue pair of B LYS 26 and A GLU 128 plays an pivotal role both in the binding of semaglutide to GLP-1 ECD and in the stabilization of the ligand-receptor complex structure [21, 3741], because the two oppositely charged residues are in interfacial contact, according to the Prodigy [30, 31] analysis of PDB entry 4ZGM [24]