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Gene expression levels were quantified by qPCR using QuantiFast SYBR Green PCR Kit (Qiagen, Chatsworth, CA, USA) and reactions were performed on a Viia TM 7 real time PCR system (Life Technologies, Gaithersburg, MD, USA)

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Then, you'll be prompted to answer a questionnaire, which asks you to disclose if you've ever enrolled in a weight loss program before, if you have family members with weight struggles, and other similar questions

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