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Nutrients absorbed in the intestine and microbial metabolites are collected via the superior and inferior mesenteric veins into the portal vein, entering the liver directly for first-pass metabolism. This unique circulatory pathway positions the liver as the first station for processing gut-derived substances ( The portal venous system carries multiple signaling molecules from the intestine

Many commonly studied ingredients such as vitamin C, glutathione precursors, niacinamide, and plant polyphenols have established safety data when taken within recommended ranges

Methanol was quantified using GC-FID and kept between 500 and 1,000 mM by constant feeding of pure methanol at manually set rates depending on the growth stage of the culture