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8) sh PPAR group: the cells transfected with shRNA duplexes targeting PPAR were cultured in DMEM for 48 h

One small handling tip: 30G needles can bend when pushing through the recessed-stopper bac vials some compounding pharmacies use if you are drawing from one of those, a 29G draw needle and a separate 31G inject needle is gentler on your hands

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Bottom middle-right, Local resolution-filtered EM maps (consensus and receptor/ECD focused refinements) displaying local resolution () coloured from highest resolution (dark blue) to lowest resolution (red)