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no therapeutic intervention trials published Cell culture Skeletal muscle myoblasts, cardiomyocytes, endothelial cells, fibroblasts, mesenchymal stem cells Ex vivo tissue preparations Isolated mitochondria, cardiac tissue preparations for respiration studies Research Limitations & Regulatory Status Critical Gaps in Current Evidence Despite over 100 published preclinical studies and growing scientific interest, MOTS-c faces substantial translational barriers that limit clinical application and necessitate cautious interpretation of its therapeutic potential

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Achievement of normal liver fat content (less than 5%) occurred in 27% of participants receiving 1 mg, 52% receiving 4 mg, 79% receiving 8 mg, and 86% receiving 12 mg at 24 weeks, compared to 0% with placebo

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