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In accord with the upregulated expression of all the ThDP-dependent dehydrogenases, transcripts of the thiamine/ThDP transporters and the major producer of ThDP from thiamine, i.e., thiamine diphosphokinase (TPK), as well as probable ThMP phosphatase ACP1, also undergo a long-term upregulation (Figure 4B)

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In some embodiments, after the first stage, the dispersed microparticles in the solvent immiscible fluid medium are isolated from the fluid medium by any convenient means of separation

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