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Binding of Cu(II) ions to ceruloplasmin Our results confirm that at pH 7.4 CuCP complex can not be demetallated with high millimolar concentrations of strong Cu(II)-chelating ligands like EDTA or DTPA in a timeframe of up to 20 h, which could be explained with three possibilities: first the thermodynamic Cu(II)-binding affinity of CuCP is much higher as compared to chelators used (for EDTA K D = 1.26 10 16 M 30