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Consistent with these findings, clinical observations in CKD cohorts have demonstrated a positive correlation between serum levels of IL-6/IL-1 and markers of erythrocyte membrane damage (e.g., increased osmotic fragility), as well as an inverse correlation with eryptosis (Mehta et al., 2017)

Feeder cells were seeded with 1.25 10 4 cells/well in a 96-well plate and cultured for 1 day in the medium including bFGF and Y-27632, and iPS cells were additionally seeded with 5000 cells/well and cultured for 1 day