Off-label use raises concerns about dosing errors, storage compliance, infection risk, and zero guarantee of safety or efficacy beyond approved regimens
For transient transfection, HT22 mouse hippocampal cells were seeded in 6-well plates at an approximate confluency of 1.0 10 6 cells/mL and then cultured for 24 h in DMEM with low glucose containing 10% fetal bovine serum, 2 mM L-glutamine, and penicillin (100 units/mL)/streptomycin (100 g/mL)
This accuracy is crucial for the integrity of clinical trials, ensuring that the data collected is relevant and valid for studies related to diabetes and obesity treatments
Mitrakou A, Ryan C, Veneman T et al (1991) Hierarchy of glycemic thresholds for counterregulatory hormone secretion, symptoms, and cerebral dysfunction