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24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants

Most protocols recommend picking one general area for consistency, then rotating specific spots within that area

It binds directly to toxins, forming a complex that can be transported out of the cell and eliminated from the body

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