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After 15 min of incubation at room temperature in the dark, cells were washed, resuspended in the binding buffer, and DAPI was added immediately before analysis on a Fortessa X-20 Cell Analyzer (BD Biosciences)

They highlight the importance of understanding the risk of joint pain when considering semaglutide for diabetes or obesity management

Elevated MDA levels compromise the structural integrity and fluidity of the sperm membrane, resulting in diminished motility and reduced viability (113, 114)