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The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74

Light may contribute to: Oxidation Photo-degradation Reduced structural stability Research storage guides commonly recommend: Storing peptides in dark environments Using opaque containers Keeping vials away from direct sunlight Storage After Reconstitution Once a peptide is mixed into solution, stability changes significantly

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