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*Correspondence: Joseph M

Isolation of INS-1-derived cell lines with robust ATP-sensitive K+channel-dependent and -independent glucose-stimulated insulin secretion

To determine the effect of the V229A substitution on the overall GLP-1R binding to cholesterol in active versus inactive conditions, cells from both sublines were labelled with PhotoClick cholesterol and exposed to UV light prior to SNAP/FLAG-hGLP-1R purification by anti-FLAG immunoprecipitation and click chemistry to fluorescently label covalently bound PhotoClick cholesterol (Figure 3C)