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Primary antibodies for western blotting Immunoblotting was performed with rabbit polyclonal anti-GCL (1:1,000) 5 , rabbit polyclonal anti-GPX1 (1:1,000) (ab59546, Abcam, Cambridge, UK), rabbit polyclonal anti-GFP (1:1,000) (ab290, Abcam), mouse monoclonal anti-GSS (1:1,000) (Sc-166882, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-GSR (Sc-133159, Santa Cruz Biotechnologies), mouse monoclonal anti-SOD2 (1:2,000) (ab16,956, Abcam), rabbit polyclonal anti-VDAC (1:1,000) (Calbiochem, Darmstadt, Germany) or mouse monoclonal anti-GAPDH (1:40,000) (glyceraldehyde-3-phosphate dehydrogenase

doi: 10.1016/j.isci.2024.110864 26 GuoLZhaoYBaiXWangXTuohetiKCaoYet al

Fistula closing may therefore be a useful self-controlling system for both skin and gastrointestinal defect healing (10-12)

These pens deliver a precise, pre-set dose, making it challenging, if not impossible, to accurately split a dose or administer it more frequently than recommended by the manufacturer