The membranes were blocked in QuickBlock Blocking Buffer for 15 min (Beyotime) and then probed with primary antibodies against fibronectin, collagen I, GFAP, -SMA, TGF1, SMAD3, phospho-Smad3 (Thr8), SMAD4, -tubulin, and -actin (Supplementary Table 2)
These include botany, morphology, microscopy, high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), liquid chromatography with mass spectrometry (LC-MS), inductively coupled plasma mass spectrometry (ICP-MS) for heavy metals testing, spectrophotometric methods (UV-Vis, FTIR), testing for microbial contamination, and other standard scientific methods
Vitamin B12 Deficiency: Many researchers believe that the standard American tests that measure serum B12 levels are far too low
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