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5 Signs That Probiotics Are Working These are five key signs that let you know your probiotic supplement is working: Improved digestionless constipation 8, diarrhea 7, belly pain 20, gas 21, bloating 17, or acid reflux or heartburn 22 Improved mood 23 and cognitive function 24 Lower stress 25 and better sleep quality 26 Less immune reactivity, including autoimmune flare-ups 27 28, allergies 29, and food sensitivities 10 Skin improvements 30 31 Im often amazed by how comprehensive probiotic supplements can be for improving gut health and overall health