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Most proteins are not stable in acidic pH, so you will want to adjust your pH back to the desired value using a concentrated base such as sodium hydroxide before using your elution buffer

10.1016/S0140-6736(68)92726-8 27 CorcoranC.JacobsT

When activated, these receptors can influence neuroinflammation, neuronal excitability, and nociceptive processing, the fundamental components of pain perception