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The best resolved classes, 233K and 346K particles of semaglutide and taspoglutide complexes respectively, were further refined to generate ECD-focused maps

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Eight MYB binding sites (MBS) of MrGST1 promoter were mutated one by one with the Fast Mutagenesis System Kit (TransGen) and then inserted into the pGreen II 0800-LUC vector to generate MrGST1mx -LUC (x stands for an integer from 1 through 8)

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