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ROS levels in the cells were detected using an ROS Assay Kit (#S0033, Beyotime) based on the DCFH-DA method

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In addition, in a cohort of women eligible for IVF treatment, women who consumed folic acid have decreased concentration of homocysteine in follicular fluid and serum, and showed improved quality and increased maturity of oocytes (53)

JCI insight 1(15):e89776