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Chronic inflammation in obesity and the metabolic syndrome

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Long term: 20 C is recommended

Culture and differentiation of primary human bronchial epithelial cells (phBEC) Normal primary human bronchial epithelial cells (phBEC) from three healthy donors were obtained from Lonza (Wokingham, UK), cultured in BEGM medium (Lonza) and differentiated at the air-liquid interface (ALI) in PneumaCult-ALI medium (Stemcell Technologies, Cologne, Germany) for up to 2833 days as described previously 67