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doi: 10.1186/s12935-019-0939-2 234 YuRZhouYShiSWangXHuangSRenY
Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (12 x 10 5 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene
Therefore, enhancing bioavailability and stability will be key in the future, although breakthroughs in once-weekly oral GLP-1 drugs are still awaited
Potential interventions include pharmacological agents ( e.g ., aminoguanidine, metformin, phenylthiazolidine, and pyridoxamine), dietary modifications ( e.g ., low-AGE diets), and regular physical exercise (81, 82)
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