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According to datasheet 1 10 6 cells were incubated with the antibody for 1 h at room temperature after washing with PBS

As the granular layer cells continue to move to the superficial layer of keratinization, mucopolysaccharides, phospholipids and other contents continue to be expelled from the membrane by particles and enter the intercellular space, which makes the adhesion between the surface layer cells firmer and can prevent the invasion of foreign materials (12)

Lin J, Liao X, Zhang J, Du G, Chen J

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