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Davinelli, S., Nielsen, M

Tirzepatide (Compounded): A dual GLP-1/GIP receptor agonist that regulates appetite, improves metabolic function, and enhances fat loss

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To isolate cardiac myocyte from C57BL/6 mice, cardiac tissue samples were digested using collagenase and maintained in media containing 10% FBS and penicillin/streptomycin, with cardiac myocytes being purified via differential applanation