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Reactive oxygen species-induced oxidative stress in the development of insulin resistance and hyperandrogenism in polycystic ovary syndrome

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Carbonyl cyanide- p -trifluoromethoxyphenylhydrazone (FCCP)-stimulated respiration was lower than ADP-stimulated respiration, a pattern observed when FCCP or substrates become limiting and previously reported in Seahorse assays using isolated mitochondria 6,14