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Gene expression profiling, data analysis and pathway analysis Total RNAs from NTAPP-treated cells or non-treated cells were purified and isolated using an RNeasy plus mini kit (Qiagen, Hilden, Germany)

Chemical synthesis and subsequent in vivo evaluation of a GIP/GLP-1MK-801 co-agonist conjugate revealed enhanced weight loss in DIO mice compared with mice treated with the GIP/GLP-1 co-agonist alone (Extended Data Fig