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Movies were recorded in hardware-binned mode (previously called counted mode on the K2 camera) with the experimental parameters listed in Table S1 using a 9-position beam-image shift acquisition pattern by custom scripts in SerialEM(Schorb et al

(2017) sugar accumulation could be casually related to cold acclimatation or they could indirectly serve as energy or carbon source for further metabolic changes related to an enhanced CI acclimatation, as previously reported in Arabidopsis (Klotke et al., 2004)