Cell culture INS-1 832/3 cells, a rat insulinoma subline with preserved incretin responses (64), and derivative INS-1 832/3 cells with endogenous rat GLP-1R deleted by CRISPR/Cas9 [INS-1 832/3 GLP-1R KO (27)], were grown in RPMI-1640 medium with 2 mM L-glutamine and 11 mM D-glucose, supplemented with 10% FBS, 1% p/s, 10 mM HEPES solution (H3537, Sigma-Aldrich), 1 mM sodium pyruvate (11360070, Thermo Fisher Scientific) and 0.05 mM -mercaptoethanol (M3148, Sigma Aldrich) in a humidified incubator at 37C and 5% CO 2
10.1016/j.heliyon.2023.e23451 Heliyon
Recalls & Warnings July 30, 2003 Maker of Weight-Loss and Cellulite Treatment Ordered to Stop False Claims and Pay Consumer Redress On July 30, 2003 the Federal Trade Commission (FTC) reported that a Canadian-based company operating in the United States under the name Bio Lab, and its president, Jean-Francois Brochu, have agreed to settle FTC charges that they deceived consumers through false and unsubstantiated advertising
J Clin Oncol 1996