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Then the biotinylate PCR products were investigated for pyrosequencing-based methylation utilizing PyroMark Q48 Advanced CpG Reagent (Qiagen cat# 974022) and commercially designed primers including SORBS1_03 (PM00042168) Chromosome 10, bp 9731953497321622, AGBL4_03 (PM00094297) Chromosome 1, bp 5048873950490934, CREB 1 (CRTC1_01) (PM00188804) Chromosome 19, bp 1879311418795233 and BDNF_01 (PM00155491) Chromosome 11, bp 2772146027723528

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