Day 10 cultured SCD erythroblasts were treated with 75 M hemin (#51280, Sigma) (dissolved in 0.25 M NaOH then neutralized with HCL to pH7.4), 50 M dimethyl fumarate (#242926, Sigma) (dissolved in DMSO), 0.5 mM (2S)-Tetrahydro-5-oxo-2-furancarboxylic acid (TFMB-L2HG) (#SML2563, Sigma) (dissolved in DMSO), 0.5 mM S-(5-Adenosyl)-L-methionine iodide (SAM) (#A4377, Sigma) (dissolved in water), and cultured under hypoxia (1% O 2 ) using Heracell 150i incubator equipped with an Oxygen (O 2 ) sensor and Nitrogen (N2) gas supply and analyzed on day 12 of culture
No one knows for sure, but evidence supports the theory that the increase in free radicals and the resulting oxidative damage is partially to blame
Combination with Other Peptides BPC-157 is particularly effective when combined with other peptides for synergistic restoration: TB-500 (Thymosin beta-4): Gold standardlocal restoration (BPC) plus systemic actin regulation and cell migration (TB)
Other booster shots that are common with these drips include vitamin B12, magnesium, vitamin D and glutathione
