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The morphologies of lyophilized hydrogels were examined by scanning electron microscopy (SEM, Hitachi S-3400N) at an accelerating voltage of 5 kV

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In this case, cells were cultured in 24-well plates at 1.2e5 cells/well in 500 l of RPMI-1640 with 10% FBS

This study investigates these properties using a p-chlorophenylalanine (PCPA) -induced insomnia model in mice, an approach akin to traditional methods evaluating sleep-promoting activities in fusion peptides