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Provided herein are methods comprising the administration of said auris formulations or compositions on or near the round window membrane via intratympanic injection

The peptide has to penetrate the outer skin barrier (stratum corneum), which limits how much active compound reaches the deeper dermal layers where collagen and elastin are produced

cells treated with the lowest concentration of 1 accumulated the most intracellular Os), though it is unclear whether this phenomenon is due to active transportation mechanism (since passive diffusion would typically increase accumulation with respect to concentration) or passive diffusion (depending on the hydrophobicity of the compound and its ability to cross cell membranes passively)

Harris ML, Titler MG, Hoffman GJ