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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

10.1016/j.redox.2020.101544 Redox Biol

D.LeBlancS

For instance, in oral diseases, infection with Porphyromonas gingivalis in patients with periodontitis induces the release of HMGB1 from host cells, which promotes osteoclastogenesis, leading to periodontal tissue destruction and bone resorption [334]