The results of studies conducted in East Asian countries indicate that highly urbanized cities, often without adequate sewage infrastructure, are characterized by the presence of microplastics in the soil at levels of 0.250.55 mg/kg which seems to be an extreme result [14]
Cell culture The human ovarian granulosa cell line KGN (Procell, Wuhan, China) was cultured in Dulbecco's modified Eagle's medium (DMEM)-F12 medium (GIBCO-BRL, Invitrogen) containing 10% fetal bovine serum (Invitrogen) and 1% penicillinstreptomycin (Invitrogen) at 37C under 5% CO
1 L-ornithine l-aspartate was successfully used to prevent brain edema in rats, but in humans, no benefit was seen compared with placebo
Upon activation, Nrf2 translocates to the nucleus, binds specifically to the Antioxidant Response Element (ARE) and induces transcription of downstream genes (Baird and Dinkova-Kostova, 2011) (Figure 2B)