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2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood

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[DOI] [PMC free article] [PubMed] [Google Scholar] [33].Brown J, Grzeskowiak L, Williamson K, Downie MR, Crowther CA

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