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Dual-luciferase assay HEK293T cells were transfected with 10 ng each of psiCHECK-2 construct along with 15 pmol of the miR-483-3p mimic or an identical amount of the control mimic with Lipofectamine 2000 (Invitrogen)

It is crucial to recognize that patients with cirrhosis often experience electrolyte imbalances, making them particularly susceptible to QT interval prolongation (4, 8)

To assess enhanced analgesia, the first assessment at time "0" was preceded by the application of morphine or saline 30 min before (time "-30 min"), by the application of haloperidol or saline 20 min (time "-20 min"), and, lastly, by the application of gastric pentadecapeptide BPC 157 or naloxone or saline (5 ml/kg i.p.) 10 min (time "-10 min")