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The plasmid system is relatively stable but has low cutting and editing efficiency, and continuous expression of Cas9 may increase off-target effects

1Thymosin 4 and cardiac protection: implication in inflammation and fibrosisPubMed / Annals of the New York Academy of Sciences 2012 2Thymosin beta 4: a multi-functional regenerative peptidePubMed / Expert Opinion on Biological Therapy 2012

But am doing far more than a year ago so doesn't really make sense