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Gather Your Supplies Before starting, make sure you have all the items youll need within reach, such as: Medication vial(s) Syringe and needle Alcohol swabs Sharps disposal container Gauze or cotton pads Having all your supplies ready ahead of time helps the process go smoothly

For the LC-MS measurement, an Agilent 6220 TOF-MS with a Dual ESI-source and a 1200 HPLC system (Agilent Technologies, Inc., Santa Clara, CA, United States) with a Hypersil Gold C18 column (1.9 m, 50 2.1 mm

Immunoprecipitation Immunoprecipitation (IP) of NHR-49::GFP was performed using either GFP-Trap Magnetic agarose beads (Cat

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