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Effects of penicillin and silymarin on liver enzymes and blood clotting factors in dogs given a boiled preparation of Amanita phalloides

Cui M, Xiao Z, Sun B, Wang Y, Zheng M, Ye L, et al

The VKDB in these cases is usually severe

Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls