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This triggers broken immune tolerance: Overactivated innate immune cells secrete pro-inflammatory cytokines, CD4 + T cells skew toward pro-inflammatory subsets (T-helper 1 [Th1] cells/Th17), T regulatory cell (Treg) function is impaired, and autoantibodies exacerbate inflammation

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A common laboratory approach is to add 2 mL of bac water to a 10mg vial, yielding a 5 mg/mL working concentration