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FIGURE 1 siRNA Knockdown of Grin1 in GLUTag Cells Increase GLP-1 Release In order to control for any possible off-target effects of MK-801which is known to also target acetylcholine receptors (nAChR), and to unequivocally confirm a direct role of NMDA receptor activity in the regulation of GLP-1 release from GLUTag cells, the essential NMDA receptor subunit, GluN1 (also called NMDAR1 or zeta-1), was knocked down by siRNA targeting of the gene Grin1, (Figure 2

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